
10
E. MonitoringPercentSleepforAllActiveChannels(SummaryTab)
The percentage sleep for each channel can be observed at any point during the recording in the
Summary panel shown in Fig. 9. The percentage is based on the sleep threshold and the
histogram formed from the accumulating data. The threshold can be changed during the
experiment, which will change these numbers accordingly. The example shown below is for
noise only channels with sleep percentages all less than 1%. Outliers can be identified here early
in the experiment and can be followed up with direct observation of the mouse or checking the
function of the sensor (e.g. ensure cable is plugged in).
Figure 9. Sleep percentage summary.
F. CurrentFileInformationandReal‐TimeSettings(Settings/Information
Tab)
After data collection has begun, current information and settings will appear on the
Settings/Information tab shown in Fig. 10. The information in the following text boxes cannot be
changed: Number of active channels, Start Time, File Path, and Active NI-DAQ Device. These
were set earlier when the data acquisition session began. The start time is stored in the data file
header so it is saved with the data to create an absolute time axis for computing statistics related
to the light and dark periods, as well as confirming the date the experiment began. The last line
can be changed and is described below.
Sleep Threshold: The classifier maps features extracted from the signal dynamics related to
sleep to greater (positive) values and those related to wake to lesser (negative) values. Since
these dynamics vary for different mice, the decision statistics will likewise shift depending on
the degree to which the mice follow the average patterns derived from the examples used during
the development of the algorithm . The sleep signal dynamics have less variability than the wake
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